![]() ![]() The key to preventing such products from entering the clinic is to improve the sensitivity of MEAs and ensure clinically relevant results. The more troubling scenario is the possibility that a product could support the development of murine embryos but still be unsuitable for clinical use. If a given product inhibits the development of murine embryos, there is little question whether that product should be used for clinical ART. However, we are not aware of any experimental data comparing the minimal inhibitory concentrations of various contaminants on murine and human embryos. The use of the murine embryo in quality control assays requires one to assume that the sensitivity of the murine embryo to various contaminants is similar to, or even greater than, that of human embryos. Īlthough the mouse embryo assay (MEA) is used to screen many products before use in human assisted reproductive technologies (ART), the clinical relevance of its results must be interpreted cautiously. As a result, all supplies that will come into contact with the embryo and/or the culture medium should be subjected to strict quality control (QC) testing, including the ability to support the growth of murine embryos. Even when the highest-quality reagents and plasticware are used, the introduction of some unknown substances (contaminants) into the culture environment is unavoidable. The effects of culture conditions on embryo viability are widely acknowledged, but controlling these conditions is not always straightforward.
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